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ProductsBack/IHC-plus(tm) CTBP1 / CTBP Antibody

IHC-plus(tm) CTBP1 / CTBP Antibody

LS-B14507
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCTBP1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) CTBP1 / CTBP Antibody
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ChrIP (1:50 - 1:200), IF (1:50 - 1:200), IHC, IHC-P (5 µg/ml), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1487
  • Target name
    CTBP1
  • Target description
    C-terminal binding protein 1
  • Target synonyms
    BARS, HADDTS, C-terminal-binding protein 1, brefeldin A-ribosylated substrate
  • Host
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of CTBP1 using anti-CTBP1 antibody (PB9425). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTBP1 antigen affinity purified polyclonal antibody (Catalog # PB9425) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTBP1 at approximately 48 kDa. The expected band size for CTBP1 is at 48 kDa.
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