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ProductsBack/IHC-plus(tm) CYLD Antibody (clone 1667CT857.3.6.1)

IHC-plus(tm) CYLD Antibody (clone 1667CT857.3.6.1)

LS-B16765
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCYLD
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) CYLD Antibody (clone 1667CT857.3.6.1)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Western Blot Predicted Molecular Weight: 107.3 kDa
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Flo (1:25), IHC-P (1:50), WB (1:4000) Western Blot Predicted Molecular Weight: 107.3 kDa
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1667CT857.3.6.1
  • Conjugate
    Unconjugated
  • Gene ID1540
  • Target name
    CYLD
  • Target description
    CYLD lysine 63 deubiquitinase
  • Target synonyms
    BRSS, CDMT, CYLD1, CYLDI, EAC, FTDALS8, MFT, MFT1, SBS, TEM, USPL2, ubiquitin carboxyl-terminal hydrolase CYLD, cylindromatosis (turban tumor syndrome), deubiquitinating enzyme CYLD, probable ubiquitin carboxyl-terminal hydrolase CYLD, ubiquitin specific peptidase like 2, ubiquitin thioesterase CYLD, ubiquitin thiolesterase CYLD, ubiquitin-specific-processing protease CYLD
  • Host
    Mouse
  • Isotype
    IgG2a
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CYLD using anti-CYLD antibody (PB9543). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Testis Tissue Lysate at 50ug, Lane 2: Rat Brain Tissue Lysate at 50ug, Lane 3: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYLD antigen affinity purified polyclonal antibody (Catalog # PB9543) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYLD at approximately 107 kDa. The expected band size for CYLD is at 107 kDa.
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