IHC-plus(tm) DAGLA Antibody (aa1031-1042)

LifeSpan BioSciences

IHC-plus(tm) DAGLA Antibody (aa1031-1042)

23

Immunohistochemistry: LS-B3123 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3123 was determined to be 5 ug/ml. Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 125kD band observed in Human Liver lysates (calculated MW of 115kD according to NP_006124.1). Recommended concentration: 0.3-1 ug/ml. Some minor background is detected and is blocked by the immunizing peptide.. IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:128000), WB (0.3 - 1 µg/ml) Immunohistochemistry: LS-B3123 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3123 was determined to be 5 ug/ml. Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 125kD band observed in Human Liver lysates (calculated MW of 115kD according to NP_006124.1). Recommended concentration: 0.3-1 ug/ml. Some minor background is detected and is blocked by the immunizing peptide.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

0.5 mg/ml

Unconjugated

DAGLA

diacylglycerol lipase alpha

C11orf11, DAGL(ALPHA), DAGLALPHA, NOC2, NSDDR, diacylglycerol lipase-alpha, DAGL-alpha, DGL-alpha, neural stem cell-derived dendrite regulator, sn1-specific diacylglycerol lipase alpha

Goat

Human

-20°C

41116161