IHC-plus(tm) DDB1 Antibody
LS-B3138
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetDDB1
Overview
- SupplierLifeSpan BioSciences
- Product NameIHC-plus(tm) DDB1 Antibody
- Delivery Days Customer23
- Application Supplier NoteImmunohistochemistry: LS-B3138 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3138 was determined to be 5 ug/ml. Immunoprecipitation: 5-10 ug/reaction. Positive control: Human HeLa cells.. ELISA (0.1 - 1 µg/ml), IHC, IHC-P (5 µg/ml), IP, WB (1 - 5 µg/ml) Immunohistochemistry: LS-B3138 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3138 was determined to be 5 ug/ml. Immunoprecipitation: 5-10 ug/reaction. Positive control: Human HeLa cells.
- ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Concentration0.5 mg/ml
- ConjugateUnconjugated
- Estimated Purity...
- Gene ID1642
- Target nameDDB1
- Target descriptiondamage specific DNA binding protein 1
- Target synonymsDDBA, UV-DDB1, WHIKERS, XAP1, XPCE, XPE, XPE-BF, DNA damage-binding protein 1, DDB p127 subunit, DNA damage-binding protein a, HBV X-associated protein 1, UV-DDB 1, UV-damaged DNA-binding factor, UV-damaged DNA-binding protein 1, XAP-1, XPE-binding factor, damage-specific DNA binding protein 1, 127kDa, xeroderma pigmentosum group E-complementing protein
- HostMouse
- IsotypeIgG1
- ReactivityHuman
- Storage Instruction2°C to 8°C or -20°C
- UNSPSC12352203






![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDB1 antibody [N1N3] (GTX100129) diluted at 1:5000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100129/GTX100129_39384_20160728_WB_shRNA_watermark_w_23053123_216.webp)