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ProductsBack/IHC-plus(tm) DFFA / ICAD / DFF45 Antibody

IHC-plus(tm) DFFA / ICAD / DFF45 Antibody

LS-B14741
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityMouse
TargetDFFA
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) DFFA / ICAD / DFF45 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    ICAD antibody can be used for detection of of ICAD by Western blot at 1 ug/mL. A 45 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 2 ug/mL. For immunofluorescence start at 10 ug/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Western Blot: 45 kDa. ELISA, IF, IHC, IHC-P (10 µg/ml), WB ICAD antibody can be used for detection of of ICAD by Western blot at 1 ug/mL. A 45 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 2 ug/mL. For immunofluorescence start at 10 ug/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Western Blot: 45 kDa
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1676
  • Target name
    DFFA
  • Target description
    DNA fragmentation factor subunit alpha
  • Target synonyms
    DFF-45, DFF1, ICAD, DNA fragmentation factor subunit alpha, DFF45, DNA fragmentation factor 45 kDa subunit, DNA fragmentation factor, 45kDa, alpha polypeptide, inhibitor of CAD
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DFFA/ICAD using anti-DFFA/ICAD antibody (A03671-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human HEPG2 whole cell lysates, Lane 4: rat stomach tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DFFA/ICAD antigen affinity purified polyclonal antibody (Catalog # A03671-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DFFA/ICAD at approximately 45KD. The expected band size for DFFA/ICAD is at 45KD.
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