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IHC-plus(tm) DIS3 Antibody

LS-B10106
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetDIS3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) DIS3 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    DIS3 antibody can be used for detection of DIS3 by Western blot at 1 - 2 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Western Blot: Predicted: 105 kDa Observed: 103 kDa. ELISA, IF (20 µg/ml), IHC, IHC-P (10 µg/ml), WB (1 - 2 µg/ml) DIS3 antibody can be used for detection of DIS3 by Western blot at 1 - 2 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Western Blot: Predicted: 105 kDa Observed: 103 kDa
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID22894
  • Target name
    DIS3
  • Target description
    DIS3 exosome endoribonuclease and 3''-5'' exoribonuclease
  • Target synonyms
    2810028N01Rik, EXOSC11, KIAA1008, RRP44, dis3p, exosome complex exonuclease RRP44, DIS3 exosome endoribonuclease and 3'-5' exoribonuclease, DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease, DIS3 mitotic control homolog, exosome component 11, mitotic control protein dis3 homolog, ribosomal RNA-processing protein 44
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DIS3 using anti-DIS3 antibody (A01736-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DIS3 antigen affinity purified polyclonal antibody (Catalog # A01736-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DIS3 at approximately 109 kDa. The expected band size for DIS3 is at 109 kDa.
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