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ProductsBack/IHC-plus(tm) DUSP6 / MKP3 Antibody (aa14-201)

IHC-plus(tm) DUSP6 / MKP3 Antibody (aa14-201)

LS-B10240
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetDUSP6
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) DUSP6 / MKP3 Antibody (aa14-201)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA (1:20000 - 1:80000), IHC, IHC-P (10 µg/ml), WB (1:1000 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1848
  • Target name
    DUSP6
  • Target description
    dual specificity phosphatase 6
  • Target synonyms
    HH19, MKP3, PYST1, dual specificity protein phosphatase 6, MAP kinase phosphatase 3, dual specificity protein phosphatase PYST1, mitogen-activated protein kinase phosphatase 3, serine/threonine specific protein phosphatase
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DUSP6 using anti-DUSP6 antibody (A02157-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DUSP6 antigen affinity purified polyclonal antibody (Catalog # A02157-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DUSP6 at approximately 50 kDa. The expected band size for DUSP6 is at 50 kDa.
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