IHC-plus(tm) FBXW7 / FBW7 Antibody (Internal)

LifeSpan BioSciences

IHC-plus(tm) FBXW7 / FBW7 Antibody (Internal)

23

Immunohistochemistry: LS-B2909 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2909 was determined to be 5 ug/ml. Immunoprecipitation: 7 ug/reaction. Positive control: Human T47D, U2-OS, MCF-7, HepG2 and HeLa cell lysates.. IHC, IHC-P (5 µg/ml), IP, WB (1 - 3 µg/ml) Immunohistochemistry: LS-B2909 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2909 was determined to be 5 ug/ml. Immunoprecipitation: 7 ug/reaction. Positive control: Human T47D, U2-OS, MCF-7, HepG2 and HeLa cell lysates.

ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

0.25 mg/ml

Unconjugated

FBXW7

F-box and WD repeat domain containing 7

AGO, CDC4, DEDHIL, FBW6, FBW7, FBX30, FBXO30, FBXW6, SEL-10, SEL10, hAgo, hCdc4, F-box/WD repeat-containing protein 7, F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase, F-box and WD-40 domain protein 7 (archipelago homolog, Drosophila), F-box protein FBW7, F-box protein FBX30, F-box protein SEL-10, archipelago, homolog of C elegans sel-10

Rabbit

IgG

Human

-20°C,2°C to 8°C

41116161