IHC-plus(tm) FFAR2 / GPR43 Antibody (C-Terminus)

LifeSpan BioSciences

IHC-plus(tm) FFAR2 / GPR43 Antibody (C-Terminus)

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Human GPR43. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except BMI1 (50%).

Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 14 ug/ml.. ELISA, IHC, IHC-P (20 µg/ml) Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 14 ug/ml.

ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

...

FFAR2

free fatty acid receptor 2

fatty acid receptor 2; FFA2R; free fatty acid activated receptor 2; free fatty acid receptor 2; GPR43; G-protein coupled receptor 43

Rabbit

Human

-20°C or -80°C,2°C to 8°C

12352203