IHC-plus(tm) FGF1 / Acidic FGF Antibody

LifeSpan BioSciences

IHC-plus(tm) FGF1 / Acidic FGF Antibody

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Immunohistochemistry: LS-B8222 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human FGF-acidic (10 ng/ml), a concentration of 1-2.5 ug/ml of this antibody is required. ELISA: To detect Human FGF-acidic by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human FGF-acidic (LS-C104591) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human FGF-acidic. Western Blot: To detect Human FGF-acidic by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human FGF-acidic is 1.5-3 ng/lane, under either reducing or non-reducing conditions.. ELISA (0.5 - 2 µg/ml), IHC, IHC-P (5 µg/ml), WB (0.1 - 0.2 µg/ml) Immunohistochemistry: LS-B8222 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human FGF-acidic (10 ng/ml), a concentration of 1-2.5 ug/ml of this antibody is required. ELISA: To detect Human FGF-acidic by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human FGF-acidic (LS-C104591) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human FGF-acidic. Western Blot: To detect Human FGF-acidic by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human FGF-acidic is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

Unconjugated

FGF1

fibroblast growth factor 1

AFGF, ECGF, ECGF-beta, ECGFA, ECGFB, FGF-1, FGF-alpha, FGFA, GLIO703, HBGF-1, HBGF1, fibroblast growth factor 1, beta-endothelial cell growth factor, endothelial cell growth factor, alpha, endothelial cell growth factor, beta, fibroblast growth factor 1 (acidic), heparin-binding growth factor 1

Rabbit

Human

-20°C,2°C to 8°C,RT

41116161