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ProductsBack/IHC-plus(tm) FGF10 Antibody (aa136-165)

IHC-plus(tm) FGF10 Antibody (aa136-165)

LS-B13752
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetFGF10
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) FGF10 Antibody (aa136-165)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:10 - 1:100), WB (1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2255
  • Target name
    FGF10
  • Target description
    fibroblast growth factor 10
  • Target synonyms
    LADD3, fibroblast growth factor 10, FGF-10, keratinocyte growth factor 2, produced by fibroblasts of urinary bladder lamina propria
  • Host
    Rabbit
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FGF10 using anti-FGF10 antibody (A01709). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat ovary tissue lysates, Lane 2: mouse lung tissue lysates, Lane 3: mouse ovary tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse NIH-3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF10 antigen affinity purified polyclonal antibody (Catalog # A01709) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF10 at approximately 20KD. The expected band size for FGF10 is at 20KD.
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