IHC-plus(tm) Filamin Antibody

LifeSpan BioSciences

IHC-plus(tm) Filamin Antibody

23

Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence. Western Blot: 1:250-1:1000. Immunoprecipitation: Suggested lysis buffer is PBS with 0.5% triton X-100 with proteinase inhibitors (note for full length filamin include calpain inhibitors). 5 microliters of antibody for. every 300-500muL of cell lysate (200-500 ug/mL total protein is suggested. Incubation is 1hour RT or overnight 4C; Protein A/G agarose beads or rabbit anti-mouse secondary capture antibody is recommended for best recovery. 4-10% acrylamide gels are. recommended for full length filamin or the 80kD fragment visualization. Immunofluorescence:1:50-1:200. ABC. Immunohistochemistry: foramlin-fixed paraffin-embedded sections. Requires HIER (citrate buffer). Positive control: Human, prostate or bovine skin.. IF (1:50 - 1:200), IHC, IHC-P (1:50), IP, WB (1:250 - 1:1000) Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence. Western Blot: 1:250-1:1000. Immunoprecipitation: Suggested lysis buffer is PBS with 0.5% triton X-100 with proteinase inhibitors (note for full length filamin include calpain inhibitors). 5 microliters of antibody for. every 300-500muL of cell lysate (200-500 ug/mL total protein is suggested. Incubation is 1hour RT or overnight 4C; Protein A/G agarose beads or rabbit anti-mouse secondary capture antibody is recommended for best recovery. 4-10% acrylamide gels are. recommended for full length filamin or the 80kD fragment visualization. Immunofluorescence:1:50-1:200. ABC. Immunohistochemistry: foramlin-fixed paraffin-embedded sections. Requires HIER (citrate buffer). Positive control: Human, prostate or bovine skin.

ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

Unconjugated

...

Mouse

IgG1

Bovine, Human

-20°C,2°C to 8°C

12352203