IHC-plus(tm) Filamin Antibody (N-Terminus)

LifeSpan BioSciences

IHC-plus(tm) Filamin Antibody (N-Terminus)

23

Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence. Western Blot: 1:500-1:2000. Immunoprecipitation: 5 ul per 300-500 ul of cell lysate (200-500 ug total protein). Recommended Lysis buffer is PBS, 05% triton X-100 with proteinase inhibitors. Note: For full-length filamin include calpain inhibitors. Incubate for 1 hour at RT or overnight at 4C. For best recovery, use Protein A/G agarose beads or rabbit anti-mouse secondary capture antibody. 4-8% acrylamide gels are recommended for full-length filamin or the 190kD fragment separation and visualization. Immunofluorescence (Frozen): 1:25-1:100. Immunohistochemistry, human-FFPE, 1:50.. IF (1:50 - 1:200), IHC, IHC-P (1:50), IP, WB (1:500 - 1:2000) Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence. Western Blot: 1:500-1:2000. Immunoprecipitation: 5 ul per 300-500 ul of cell lysate (200-500 ug total protein). Recommended Lysis buffer is PBS, 05% triton X-100 with proteinase inhibitors. Note: For full-length filamin include calpain inhibitors. Incubate for 1 hour at RT or overnight at 4C. For best recovery, use Protein A/G agarose beads or rabbit anti-mouse secondary capture antibody. 4-8% acrylamide gels are recommended for full-length filamin or the 190kD fragment separation and visualization. Immunofluorescence (Frozen): 1:25-1:100. Immunohistochemistry, human-FFPE, 1:50.

ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

Unconjugated

Mouse

IgG1

Chicken, Guinea Pig, Human, Mouse, Rabbit, Rat

-20°C,2°C to 8°C

41116161