IHC-plus(tm) FZD5 / Frizzled 5 Antibody (N-Terminus)

LifeSpan BioSciences

IHC-plus(tm) FZD5 / Frizzled 5 Antibody (N-Terminus)

23

Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 7 ug/ml.. ELISA, IHC, IHC-P (7 µg/ml) Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 7 ug/ml.

ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

FZD5

frizzled class receptor 5

C2orf31, HFZ5, MCOPCB11, frizzled-5, Wnt receptor, frizzled 5, seven transmembrane spanning receptor, frizzled family receptor 5, fz-5, fzE5, seven-transmembrane receptor frizzled-5

Rabbit

Bovine, Canine, Human, Monkey, Porcine, Rabbit

-20°C or -80°C,2°C to 8°C

41116161