IHC-plus(tm) GFAP Antibody

LifeSpan BioSciences

IHC-plus(tm) GFAP Antibody

23

Recognizes porcine glial fibrillary acidic protein (GFAP) at 51-52kD. Species cross-reactivity: human, rat and chicken.

Immunohistochemistry: LS-B2801 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2801 was determined to be 1-2 ug/ml. Immunoprecipitation (Native and denatured): 2 ug/mg protein lysate (Use Protein G). Western Blot: 1-2 ug/ml for 2 hrs at RT. Positive control: Astrocytoma, IMR5 and brain cells.. IF, IHC, IHC-P (1 - 2 µg/ml), IP (2 µg/tst), WB (1 - 2 µg/ml) Immunohistochemistry: LS-B2801 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2801 was determined to be 1-2 ug/ml. Immunoprecipitation (Native and denatured): 2 ug/mg protein lysate (Use Protein G). Western Blot: 1-2 ug/ml for 2 hrs at RT. Positive control: Astrocytoma, IMR5 and brain cells.

ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

0.1 mg/ml

Unconjugated

...

GFAP

glial fibrillary acidic protein

ALXDRD; glial fibrillary acidic protein

Mouse

IgG1

Chicken, Human, Porcine, Rat

2°C to 8°C or -20°C

12352203