IHC-plus(tm) GLI2 Antibody (Internal)

LifeSpan BioSciences

IHC-plus(tm) GLI2 Antibody (Internal)

23

Immunohistochemistry: LS-B75 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B75 was determined to be 5 ug/ml.. ELISA (1:15000 - 1:60000), IHC, IHC-P (5 µg/ml), WB (1:500 - 1:2000) Immunohistochemistry: LS-B75 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B75 was determined to be 5 ug/ml.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

GLI2

GLI family zinc finger 2

CJS, HPE9, PHS2, THP1, THP2, zinc finger protein GLI2, GLI family zinc finger protein 2, GLI-Kruppel family member GLI2, glioma-associated oncogene family zinc finger 2, oncogene GLI2, tax helper protein 1, tax helper protein 2, tax-responsive element-2 holding protein, tax-responsive element-25-bp sequence binding protein

Rabbit

IgG

Mouse

-20°C,2°C to 8°C

41116161