IHC-plus(tm) GLI3 Antibody

LifeSpan BioSciences

IHC-plus(tm) GLI3 Antibody

23

Immunohistochemistry: LS-B73 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B73 was determined to be 1.25 ug/ml.. ELISA (1:6000 - 1:30000), IF, IHC, IHC-P (1.25 µg/ml), WB (1:500 - 1:2000) Immunohistochemistry: LS-B73 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B73 was determined to be 1.25 ug/ml.

ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

...

GLI3

GLI family zinc finger 3

ACLS, GCPS, GLI3-190, GLI3FL, PAP-A, PAPA, PAPA1, PAPB, PHS, PPDIV, transcriptional activator GLI3, GLI-Kruppel family member GLI3, glioma-associated oncogene family zinc finger 3, oncogene GLI3, zinc finger protein GLI3

Rabbit

IgG

Avian, Canine, Chicken, Human, Monkey, Primate, Xenopus

-20°C,2°C to 8°C

12352203