IHC-plus(tm) GPR149 / PGR10 Antibody (Cytoplasmic Domain)

LifeSpan BioSciences

IHC-plus(tm) GPR149 / PGR10 Antibody (Cytoplasmic Domain)

23

Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 20 ug/ml.. ELISA, IHC, IHC-P (20 µg/ml) Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 20 ug/ml.

ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

GPR149

G protein-coupled receptor 149

IEDA, PGR10, R35, probable G-protein coupled receptor 149, G protein-coupled receptor PGR10

Rabbit

Human

-20°C or -80°C,2°C to 8°C

41116161