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ProductsBack/IHC-plus(tm) HIWI2 / PIWIL4 Antibody

IHC-plus(tm) HIWI2 / PIWIL4 Antibody

LS-B14886
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Rat
TargetPIWIL4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) HIWI2 / PIWIL4 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 52kDa/56kDa/96kDa, while the observed MW by Western blot was 110kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IF (1:50 - 1:200), IHC (1:50 - 1:200), IHC-P (1:200), WB (1:500 - 1:2000) The predicted MW is 52kDa/56kDa/96kDa, while the observed MW by Western blot was 110kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID143689
  • Target name
    PIWIL4
  • Target description
    piwi like RNA-mediated gene silencing 4
  • Target synonyms
    HIWI2, MIWI2, piwi-like protein 4, piwi-like 4, testis tissue sperm-binding protein Li 85P
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PIWIL4/PIWI using anti-PIWIL4/PIWI antibody (A06797-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human PANC-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIWIL4/PIWI antigen affinity purified polyclonal antibody (Catalog # A06797-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIWIL4/PIWI at approximately 97 kDa. The expected band size for PIWIL4/PIWI is at 97 kDa.
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