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ProductsBack/IHC-plus(tm) HMMR / CD168 / RHAMM Antibody

IHC-plus(tm) HMMR / CD168 / RHAMM Antibody

LS-B7037
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHMMR
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) HMMR / CD168 / RHAMM Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    RHAMM antibody can be used for detection of RHAMM by Western blot at 0.5 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 5 ug/mL. Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:. ELISA, IF, IHC, IHC-P (5 µg/ml), WB (0.5 - 1 µg/ml) RHAMM antibody can be used for detection of RHAMM by Western blot at 0.5 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 5 ug/mL. Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3161
  • Target name
    HMMR
  • Target description
    hyaluronan mediated motility receptor
  • Target synonyms
    CD168, IHABP, RHAMM, hyaluronan mediated motility receptor, intracellular hyaluronic acid-binding protein, receptor for hyaluronan-mediated motility
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD168/HMMR using anti-CD168/HMMR antibody (A05056-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human T-47D whole cell lysates, Lane 5: human Raji whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD168/HMMR antigen affinity purified polyclonal antibody (Catalog # A05056-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD168/HMMR at approximately 84KD. The expected band size for CD168/HMMR is at 84KD.
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