IHC-plus(tm) HUS1B Antibody

LifeSpan BioSciences

IHC-plus(tm) HUS1B Antibody

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A BLAST analysis indicates 100% homology of the immunizing sequence with Hus1B protein from human. Partial reactivity with mouse Hus1B is not likely based on low (~64%, 9/14) sequence homology. Reactivity against homologues from other sources is not known

Immunohistochemistry: LS-B376 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B376 was determined to be 5 ug/ml.. ELISA (1:20000 - 1:100000), IHC, IHC-P (5 µg/ml), IP (1:100), WB (1:500 - 1:5000) Immunohistochemistry: LS-B376 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B376 was determined to be 5 ug/ml.

ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

...

HUS1B

HUS1 checkpoint clamp component B

checkpoint protein HUS1B; hHUS1B; HUS1 checkpoint homolog b

Rabbit

IgG

Human, Mouse, Rat

2°C to 8°C or -20°C

12352203