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ProductsBack/IHC-plus(tm) IDH3G Antibody (aa337-350)

IHC-plus(tm) IDH3G Antibody (aa337-350)

LS-B9541
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Hamster, Human, Monkey, Mouse, Porcine, Rat
TargetIDH3G
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) IDH3G Antibody (aa337-350)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 38kD band observed in lysates of cell line MCF7 and of Human, Mouse and Rat Heart (calculated MW of 41.5kD according to NP_777358.1). Recommended concentration: 0.5-2 ug/ml.. IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:16000), WB (0.5 - 2 µg/ml) Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 38kD band observed in lysates of cell line MCF7 and of Human, Mouse and Rat Heart (calculated MW of 41.5kD according to NP_777358.1). Recommended concentration: 0.5-2 ug/ml.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3421
  • Target name
    IDH3G
  • Target description
    isocitrate dehydrogenase (NAD(+)) 3 non-catalytic subunit gamma
  • Target synonyms
    H-IDHG, RP99, isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial, IDH-gamma, NAD (H)-specific isocitrate dehydrogenase gamma subunit, NAD(+)-specific ICDH subunit gamma, NAD+-specific ICDH, isocitrate dehydrogenase (NAD(+)) 3 gamma, isocitrate dehydrogenase 3 (NAD(+)) gamma, isocitrate dehydrogenase 3 (NAD+) gamma, isocitrate dehydrogenase 3 gamma, isocitrate dehydrogenase, NAD(+)-specific, mitochondrial, gamma subunit, isocitric dehydrogenase subunit gamma
  • Host
    Goat
  • Reactivity
    Bovine, Canine, Equine, Hamster, Human, Monkey, Mouse, Porcine, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of IDH3G using anti-IDH3G antibody (A10370-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: human U251 whole cell lysates, Lane 6: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDH3G antigen affinity purified polyclonal antibody (Catalog # A10370-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IDH3G at approximately 40 kDa. The expected band size for IDH3G is at 43 kDa.
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