IHC-plus(tm) IFN Gamma / Interferon Gamma Antibody

LifeSpan BioSciences

IHC-plus(tm) IFN Gamma / Interferon Gamma Antibody

23

Immunohistochemistry: LS-B7487 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IFN-gamma (10 ng/ml), a concentration of 0.13-0.20 ug/ml of this antibody is required. ELISA: To detect Human IFN-gamma by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IFN-gamma (LS-C104708) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IFN-gamma. Western Blot: To detect Human IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-gamma is 1.5-3 ng/lane, under either reducing or non-reducing conditions.. ELISA (0.5 - 2 µg/ml), IHC, IHC-P (5 µg/ml), WB (0.1 - 0.2 µg/ml) Immunohistochemistry: LS-B7487 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IFN-gamma (10 ng/ml), a concentration of 0.13-0.20 ug/ml of this antibody is required. ELISA: To detect Human IFN-gamma by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IFN-gamma (LS-C104708) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IFN-gamma. Western Blot: To detect Human IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-gamma is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

Unconjugated

IFNG

interferon gamma

IFG, IFI, IMD69, interferon gamma, IFN-gamma, immune interferon

Rabbit

IgG

Human

-20°C,2°C to 8°C,RT

41116161