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IHC-plus(tm) IL27 Antibody

LS-B2719
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetIL27
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) IL27 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    IL-27 antibody can be used for the detection of IL-27 by Western blot at 2 - 4 ug/mL. Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Western Blot:
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IHC, IHC-P (5 µg/ml), WB (2 - 4 µg/ml) IL-27 antibody can be used for the detection of IL-27 by Western blot at 2 - 4 ug/mL. Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Western Blot:
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID246778
  • Target name
    IL27
  • Target description
    interleukin 27
  • Target synonyms
    IL-27, IL-27A, IL27A, IL27p28, IL30, p28, interleukin-27 subunit alpha, IL-27 p28 subunit, IL-27 subunit alpha, IL-27-A, IL27-A, interleukin-30
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA127030(IL27 Antibody) at dilution 1/15, on the right is treated with fusion protein. (Original magnification: x200)
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Figure 1. Western blot analysis of IL27 using anti-IL27 antibody (A00857-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: recombinant human IL27 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL27 antigen affinity purified polyclonal antibody (Catalog # A00857-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL27 at approximately 60KD. The expected band size for IL27 is at 50KD.
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