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ProductsBack/IHC-plus(tm) KIF11 / EG5 Antibody

IHC-plus(tm) KIF11 / EG5 Antibody

LS-B14243
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetKIF11
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) KIF11 / EG5 Antibody
  • Delivery Days Customer
    23
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ICC (1:200), IHC, IHC-P (1:50), IP (1:500), WB (1:1000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID3832
  • Target name
    KIF11
  • Target description
    kinesin family member 11
  • Target synonyms
    EG5, HKSP, KNSL1, MCLMR, TRIP5, kinesin-like protein KIF11, TR-interacting protein 5, TRIP-5, kinesin-like protein 1, kinesin-like spindle protein HKSP, kinesin-related motor protein Eg5, thyroid receptor-interacting protein 5
  • Host
    Mouse
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Eg5/KIF11 using anti-Eg5/KIF11 antibody (A01754-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Daudi whole cell lysates, Lane 5: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eg5/KIF11 antigen affinity purified polyclonal antibody (Catalog # A01754-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Eg5/KIF11 at approximately 130 kDa. The expected band size for Eg5/KIF11 is at 119 kDa.
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