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ProductsBack/IHC-plus(tm) Kinesin 5A / KIF5A Antibody

IHC-plus(tm) Kinesin 5A / KIF5A Antibody

LS-B2714
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetKIF5A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) Kinesin 5A / KIF5A Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    KIF5 antibody can be used for detection of KIF5 by Western blot at 0.5 - 1 ug/mL. Antibody can also be used for immunocytochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 105, 114 kDa Observed: 118 kDa. ELISA, ICC, IF, IHC, IHC-P (5 µg/ml), WB (0.5 - 1 µg/ml) KIF5 antibody can be used for detection of KIF5 by Western blot at 0.5 - 1 ug/mL. Antibody can also be used for immunocytochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 105, 114 kDa Observed: 118 kDa
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3798
  • Target name
    KIF5A
  • Target description
    kinesin family member 5A
  • Target synonyms
    ALS25, D12S1889, MY050, NEIMY, NKHC, SPG10, kinesin heavy chain isoform 5A, KIF5A variant protein, kinesin heavy chain neuron-specific 1, kinesin, heavy chain, neuron-specific, neuron-specific kinesin heavy chain, neuronal kinesin heavy chain
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KIF5A using anti-KIF5A antibody (A03635-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIF5A antigen affinity purified polyclonal antibody (Catalog # A03635-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIF5A at approximately 117 kDa. The expected band size for KIF5A is at 117 kDa.
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