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ProductsBack/IHC-plus(tm) KISS1R / GPR54 Antibody (C-Terminus)

IHC-plus(tm) KISS1R / GPR54 Antibody (C-Terminus)

LS-A1928
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetKISS1R
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) KISS1R / GPR54 Antibody (C-Terminus)
  • Delivery Days Customer
    23
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (3 - 5 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID84634
  • Target name
    KISS1R
  • Target description
    KISS1 receptor
  • Target synonyms
    AXOR12, CPPB1, GPR54, HH8, HOT7T175, KISS-1R, kiSS-1 receptor, G protein-coupled receptor 54, G-protein coupled receptor OT7T175, hypogonadotropin-1, kisspeptins receptor, metastin receptor
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KiSS1 receptor using anti-KiSS1 receptor antibody (A01364-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human WISH whole cell lysates, Lane 2: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KiSS1 receptor antigen affinity purified polyclonal antibody (Catalog # A01364-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KiSS1 receptor at approximately 43KD. The expected band size for KiSS1 receptor is at 43KD.
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Lane 1: Mouse Spinal cord lysates; Lane 2: Rat Spinal cord lysates probed with KiSS1 receptor Polyclonal Antibody, Unconjugated (bs-2501R) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
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