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ProductsBack/IHC-plus(tm) KLK3 / PSA Antibody

IHC-plus(tm) KLK3 / PSA Antibody

LS-B16928
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetKLK3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) KLK3 / PSA Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    This antibody is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IHC-P (5 µg/ml), WB This antibody is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    9.82 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    >95%
  • Gene ID354
  • Target name
    KLK3
  • Target description
    kallikrein related peptidase 3
  • Target synonyms
    APS, KLK2A1, PSA, hK3, prostate-specific antigen, P-30 antigen, gamma-seminoprotein, kallikrein-3, semenogelase, seminin
  • Host
    Goat
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Prostate Specific Antigen/KLK3 using anti-Prostate Specific Antigen/KLK3 antibody (A01505-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human LNCAP whole cell lysates, Lane 2: human LNCAP whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prostate Specific Antigen/KLK3 antigen affinity purified polyclonal antibody (Catalog # A01505-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prostate Specific Antigen/KLK3 at approximately 34KD. The expected band size for Prostate Specific Antigen/KLK3 is at 34KD.
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