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ProductsBack/IHC-plus(tm) LEO1 Antibody (phospho-Ser10)

IHC-plus(tm) LEO1 Antibody (phospho-Ser10)

LS-B4804
LifeSpan BioSciences
ApplicationsDot Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetLEO1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) LEO1 Antibody (phospho-Ser10)
  • Delivery Days Customer
    23
  • Applications
    Dot Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    DB (1:500), IHC, IHC-P (5 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID123169
  • Target name
    LEO1
  • Target description
    LEO1 component of Paf1/RNA polymerase II complex
  • Target synonyms
    RDL, RNA polymerase-associated protein LEO1, LEO1 homolog, Paf1/RNA polymerase II complex component, Leo1 Paf1/RNA polymerase II complex component, Leo1, Paf1/RNA polymerase II complex component, homolog, replicative senescence down-regulated leo1-like protein
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LEO1 using anti-LEO1 antibody (A06433-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human RT4 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LEO1 antigen affinity purified polyclonal antibody (Catalog # A06433-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LEO1 at approximately 105 kDa. The expected band size for LEO1 is at 75 kDa.
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