IHC-plus(tm) Leptin Antibody

LifeSpan BioSciences

IHC-plus(tm) Leptin Antibody

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The antibody will recognize recombinant and native 16 kDa leptin from mouse and human. Reactivity with leptin from other sources is unknown.

Immunohistochemistry: LS-B1459 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1459 was determined to be 5 ug/ml.. ELISA (1:1000 - 1:5000), IHC, IHC-P (5 µg/ml), WB (1:500 - 1:2000) Immunohistochemistry: LS-B1459 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1459 was determined to be 5 ug/ml.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

85 mg/ml

Unconjugated

LEP

leptin

LEPD; leptin; leptin (murine obesity homolog); leptin (obesity homolog, mouse); OB; obese protein; obese, mouse, homolog of; obesity factor; OBS

Rabbit

Human, Mouse

2°C to 8°C or -20°C

12352203