IHC-plus(tm) Leptin Antibody

LifeSpan BioSciences

IHC-plus(tm) Leptin Antibody

23

Immunohistochemistry: LS-B8192 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect recombinant human Leptin when used with biotinylated antigen affinity purified anti-human Leptin as the detection antibody at a concentration of approximately 0.5-1 ug/ml. Western Blot: To detect hLeptin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLeptin is 2-4 ng/lane, under non-reducing conditions.. ELISA (12 - 16 µg/ml), IHC, IHC-P (10 µg/ml), WB (0.2 - 0.4 µg/ml) Immunohistochemistry: LS-B8192 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect recombinant human Leptin when used with biotinylated antigen affinity purified anti-human Leptin as the detection antibody at a concentration of approximately 0.5-1 ug/ml. Western Blot: To detect hLeptin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLeptin is 2-4 ng/lane, under non-reducing conditions.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

1 mg/ml

Unconjugated

...

LEP

leptin

LEPD, OB, OBS, leptin, leptin (murine obesity homolog), leptin (obesity homolog, mouse), obese protein, obese, mouse, homolog of, obesity factor

Mouse

IgG1

Human

-20°C,2°C to 8°C,RT

12352203