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IHC-plus(tm) LSR / LISCH7 Antibody

LS-B15776
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetLSR
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) LSR / LISCH7 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 54kDa/64kDa/65kDa/69kDa/71kDa, while the observed MW by Western blot was 60kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC-P (1:200), WB (1:500 - 1:2000) The predicted MW is 54kDa/64kDa/65kDa/69kDa/71kDa, while the observed MW by Western blot was 60kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    4.82 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID51599
  • Target name
    LSR
  • Target description
    lipolysis stimulated lipoprotein receptor
  • Target synonyms
    ILDR3, LISCH7, lipolysis-stimulated lipoprotein receptor, LISCH protein, angulin-1, immunoglobulin-like domain containing receptor 3, lipolysis-stimulated remnant, liver-specific bHLH-Zip transcription factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of LSR using anti-LSR antibody (A02742-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LSR antigen affinity purified polyclonal antibody (Catalog # A02742-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LSR at approximately 68 kDa. The expected band size for LSR is at 71 kDa.
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