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ProductsBack/IHC-plus(tm) MIP2 / GRO2 / CXCL2 Antibody

IHC-plus(tm) MIP2 / GRO2 / CXCL2 Antibody

LS-B15746
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCXCL2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) MIP2 / GRO2 / CXCL2 Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IHC-P (10 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2920
  • Target name
    CXCL2
  • Target description
    C-X-C motif chemokine ligand 2
  • Target synonyms
    CINC-2a, GRO2, GROb, MGSA-b, MIP-2a, MIP2, MIP2A, SCYB2, C-X-C motif chemokine 2, GRO2 oncogene, MGSA beta, MIP2-alpha, chemokine (C-X-C motif) ligand 2, gro-beta, growth-regulated protein beta, macrophage inflammatory protein 2-alpha, melanoma growth stimulatory activity beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CXCL2 using anti-CXCL2 antibody (PB9857). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human CXCL2 protein 0.5ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL2 antigen affinity purified polyclonal antibody (Catalog # PB9857) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL2 at approximately 11 kDa. The expected band size for CXCL2 is at 11 kDa.
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