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IHC-plus(tm) MTTP / MTP Antibody

LS-B15878
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMTTP
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) MTTP / MTP Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    The predicted MW is 16kDa/99kDa, while the observed MW by Western blot was 99kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IF (1:50 - 1:200), IHC-P (1:200), WB (1:500 - 1:2000) The predicted MW is 16kDa/99kDa, while the observed MW by Western blot was 99kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4547
  • Target name
    MTTP
  • Target description
    microsomal triglyceride transfer protein
  • Target synonyms
    ABL, MTP, microsomal triglyceride transfer protein large subunit, microsomal triglyceride transfer protein (large polypeptide, 88kDa)
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of MTTP using anti-MTTP antibody (A01715-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEPG2 whole cell lysates, Lane 2: monkey liver tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: rat small intestine tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTTP antigen affinity purified polyclonal antibody (Catalog # A01715-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTTP at approximately 99KD. The expected band size for MTTP is at 99KD.
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