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ProductsBack/IHC-plus(tm) MYDGF / SF20 Antibody (aa32-173)

IHC-plus(tm) MYDGF / SF20 Antibody (aa32-173)

LS-B15251
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetMYDGF
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) MYDGF / SF20 Antibody (aa32-173)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IHC, IHC-P (10 µg/ml), WB (1:200 - 1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.73 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID56005
  • Target name
    MYDGF
  • Target description
    myeloid derived growth factor
  • Target synonyms
    C19orf10, EUROIMAGE1875335, IL25, IL27, IL27w, R33729_1, SF20, myeloid-derived growth factor, UPF0556 protein C19orf10, interleukin 27 working designation, interleukin-25, stromal cell-derived growth factor SF20
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of C19orf10/MYDGF using anti-C19orf10/MYDGF antibody (A08602). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human U251 whole cell lysates, Lane 6: human THP-1 whole cell lysates, Lane 7: human A431 whole cell lysates, Lane 8: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C19orf10/MYDGF antigen affinity purified polyclonal antibody (Catalog # A08602) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C19orf10/MYDGF at approximately 15 kDa. The expected band size for C19orf10/MYDGF is at 19 kDa.
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