IHC-plus(tm) NACA Antibody (C-Terminus)

LifeSpan BioSciences

IHC-plus(tm) NACA Antibody (C-Terminus)

23

Immunohistochemistry: LS-B2470 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2470 was determined to be 5 ug/ml. Positive control: Fetal mouse brain, rat brain homogenates and human NGP96 cell lysate.. IHC, IHC-P (5 µg/ml), WB (1 - 2 µg/ml) Immunohistochemistry: LS-B2470 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2470 was determined to be 5 ug/ml. Positive control: Fetal mouse brain, rat brain homogenates and human NGP96 cell lysate.

Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

0.25 mg/ml

Unconjugated

NACA

nascent polypeptide associated complex subunit alpha

HSD48, NAC-alpha, NACA1, skNAC, nascent polypeptide-associated complex subunit alpha, alpha-NAC, muscle-specific form, nascent-polypeptide-associated complex alpha polypeptide

Rabbit

IgG

Human, Mouse, Rat

-20°C,2°C to 8°C

41116161