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ProductsBack/IHC-plus(tm) NAF-1 / CISD2 Antibody

IHC-plus(tm) NAF-1 / CISD2 Antibody

LS-B10097
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCISD2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) NAF-1 / CISD2 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    CISD2 antibody was raised against a 15 amino acid peptide near the center of human CISD2. Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Western Blot: Predicted: 15 kDa Observed: 15 kDa. ELISA, IF (20 µg/ml), IHC, IHC-P (20 µg/ml), WB (1 - 2 µg/ml) CISD2 antibody was raised against a 15 amino acid peptide near the center of human CISD2. Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Western Blot: Predicted: 15 kDa Observed: 15 kDa
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID493856
  • Target name
    CISD2
  • Target description
    CDGSH iron sulfur domain 2
  • Target synonyms
    ERIS, Miner1, NAF-1, WFS2, ZCD2, CDGSH iron-sulfur domain-containing protein 2, endoplasmic reticulum intermembrane small protein, mitoNEET-related 1 protein, nutrient-deprivation autophagy factor-1, zinc finger, CDGSH-type domain 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CISD2 using anti-CISD2 antibody (A06387-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human HELA whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: monkey kidney tissue lysates, Lane 5: human SW620 whole cell lysates, Lane 6: human Raji whole cell lysates, Lane 7: rat kidney tissue lysates, Lane 8: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CISD2 antigen affinity purified polyclonal antibody (Catalog # A06387-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CISD2 at approximately 15KD. The expected band size for CISD2 is at 15KD.
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