More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/IHC-plus(tm) NCALD / Neurocalcin Delta Antibody

IHC-plus(tm) NCALD / Neurocalcin Delta Antibody

LS-B16977
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNCALD
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) NCALD / Neurocalcin Delta Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 22kDa, while the observed MW by Western blot was 18kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IF (1:50 - 1:100), IHC-P (5 µg/ml), WB (1:500 - 1:2000) The predicted MW is 22kDa, while the observed MW by Western blot was 18kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    9.32 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID83988
  • Target name
    NCALD
  • Target description
    neurocalcin delta
  • Target synonyms
    neurocalcin-delta
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Western blot All lanes: NCALD antibody at 9.32 ug/ml + Mouse brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa
Product group Antibodies
Cusabio
NCALD AntibodyCSB-PA015510DSR1HU
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetNCALD
  • SizePrice
Product group Antibodies
Assay Genie
NCALD AntibodyCAB8000
ReactivityHuman
TargetNCALD
  • SizePrice
Figure 1. Western blot analysis of NCALD using anti-NCALD antibody (A09890-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCALD antigen affinity purified polyclonal antibody (Catalog # A09890-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCALD at approximately 22 kDa. The expected band size for NCALD is at 22 kDa.
Product group Antibodies
Boster Bio
Anti-NCALD Antibody Picoband(r)A09890-2-CARRIER-FREE
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetNCALD
  • SizePrice
Anti-NCALD Antibody
Product group Antibodies
Antibodies.com
Anti-NCALD AntibodyA16033
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
RayBiotech
Anti-NCALD Antibody144-08000
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetNCALD
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
NCALD / Neurocalcin Delta AntibodyLS-C676122
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetNCALD
  • SizePrice
Neurocalcin delta antibody detects Neurocalcin delta protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Neurocalcin delta antibody (GTX121264) diluted at 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Product group Antibodies
GeneTex
NCALD antibodyGTX121264
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetNCALD
  • SizePrice