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ProductsBack/IHC-plus(tm) NCL / Nucleolin Antibody (clone 4E2)

IHC-plus(tm) NCL / Nucleolin Antibody (clone 4E2)

LS-B11335
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetNCL
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) NCL / Nucleolin Antibody (clone 4E2)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Flo, ICC, IHC, IHC-P (10 µg/ml), WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4E2
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4691
  • Target name
    NCL
  • Target description
    nucleolin
  • Target synonyms
    C23, Nsr1, nucleolin
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NCL using anti-NCL antibody (A00228-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCL antigen affinity purified polyclonal antibody (Catalog # A00228-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCL at approximately 100-110 kDa. The expected band size for NCL is at 63 kDa.
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