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ProductsBack/IHC-plus(tm) NCR3LG1 / B7H6 Antibody (aa389-415)

IHC-plus(tm) NCR3LG1 / B7H6 Antibody (aa389-415)

LS-B12849
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetNCR3LG1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) NCR3LG1 / B7H6 Antibody (aa389-415)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:100), WB (1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID374383
  • Target name
    NCR3LG1
  • Target description
    natural killer cell cytotoxicity receptor 3 ligand 1
  • Target synonyms
    B7-H6, B7H6, DKFZp686O24166, natural cytotoxicity triggering receptor 3 ligand 1, B7 homolog 6, putative Ig-like domain-containing protein DKFZp686O24166/DKFZp686I21167
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of B7H6/NCR3LG1 using anti-B7H6/NCR3LG1 antibody (A09747-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-B7H6/NCR3LG1 antigen affinity purified polyclonal antibody (A09747-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for B7H6/NCR3LG1 at approximately 90 kDa. The expected band size for B7H6/NCR3LG1 is at 51 kDa.
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