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ProductsBack/IHC-plus(tm) NDRG2 Antibody (aa25-39)

IHC-plus(tm) NDRG2 Antibody (aa25-39)

LS-B9230
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityEquine, Hamster, Human, Mouse, Rabbit, Rat
TargetNDRG2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) NDRG2 Antibody (aa25-39)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 45kD band observed in Human Brain (Temporal Cortex), Mouse and Rat Brain lysates (calculated MW of 40.8kD according to NP_963293.1). Recommended concentration: 0.1-0.3 ug/ml.. IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:16000), WB (0.1 - 0.3 µg/ml) Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 45kD band observed in Human Brain (Temporal Cortex), Mouse and Rat Brain lysates (calculated MW of 40.8kD according to NP_963293.1). Recommended concentration: 0.1-0.3 ug/ml.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID57447
  • Target name
    NDRG2
  • Target description
    NDRG family member 2
  • Target synonyms
    SYLD, protein NDRG2, N-myc downstream regulator 2, N-myc downstream-regulated gene 2 protein, NDR1-related protein NDR2, cytoplasmic protein Ndr1, syld709613 protein
  • Host
    Goat
  • Reactivity
    Equine, Hamster, Human, Mouse, Rabbit, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NDRG2 using anti-NDRG2 antibody (PB9672). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: Mouse Brain Tissue Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDRG2 antigen affinity purified polyclonal antibody (Catalog # PB9672) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDRG2 at approximately 41 kDa. The expected band size for NDRG2 is at 41 kDa.
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