IHC-plus(tm) NET1 / ARHGEF8 Antibody

LifeSpan BioSciences

IHC-plus(tm) NET1 / ARHGEF8 Antibody

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Western blot analysis of NET1 expression in Jurkat (A), HEK293T (B) whole cell lysates. Immunofluorescent analysis of NET1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.. ICC (1:50 - 1:200), IF (1:50 - 1:200), IHC-P (1:100), WB (1:500 - 1:2000) Western blot analysis of NET1 expression in Jurkat (A), HEK293T (B) whole cell lysates. Immunofluorescent analysis of NET1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

NET1

neuroepithelial cell transforming 1

ARHGEF8, NET1A, neuroepithelial cell-transforming gene 1 protein, Rho guanine nucleotide exchange factor (GEF) 8, epididymis secretory sperm binding protein, guanine nucleotide regulatory protein (oncogene), neuroepithelioma transforming gene 1, p65 Net1 proto-oncogene protein, small GTP-binding protein regulator

Rabbit

Human, Mouse, Rat

-20°C

41116161