IHC-plus(tm) NRG1 / Heregulin / Neuregulin Antibody

LifeSpan BioSciences

IHC-plus(tm) NRG1 / Heregulin / Neuregulin Antibody

23

Immunohistochemistry: LS-B7486 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human Heregulin-beta1 (1 ng/ml), a concentration of 0.7-1.1 ug/ml of this antibody is required. ELISA: To detect Human Heregulin-beta1 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Heregulin-beta1(LS-C104552) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human Heregulin-beta1. Western Blot: To detect Human Heregulin-beta1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human Heregulin-beta1 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.. ELISA (0.5 - 2 µg/ml), IHC, IHC-P (5 µg/ml), WB (0.1 - 0.2 µg/ml) Immunohistochemistry: LS-B7486 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human Heregulin-beta1 (1 ng/ml), a concentration of 0.7-1.1 ug/ml of this antibody is required. ELISA: To detect Human Heregulin-beta1 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Heregulin-beta1(LS-C104552) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human Heregulin-beta1. Western Blot: To detect Human Heregulin-beta1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human Heregulin-beta1 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

NRG1

neuregulin 1

ARIA, GGF, GGF2, HGL, HRG, HRG1, HRGA, MST131, MSTP131, NDF, NRG1-IT2, SMDF, pro-neuregulin-1, membrane-bound isoform, NRG1 class VII isoform alpha 2a, NRG1 class VII isoform alpha 2b, NRG1 class VII isoform alpha beta 2a, NRG1 class VII isoform alpha beta 3, NRG1 class VII isoform beta 2a, NRG1 class VII isoform beta 3, acetylcholine receptor-inducing activity, glial growth factor 2, heregulin, alpha (45kD, ERBB2 p185-activator), neu differentiation factor, pro-NRG1, sensory and motor neuron derived factor

Rabbit

IgG

Human

-20°C,2°C to 8°C,RT

41116161