IHC-plus(tm) ORAI3 Antibody (N-Terminus)

LifeSpan BioSciences

IHC-plus(tm) ORAI3 Antibody (N-Terminus)

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Immunohistochemistry: LS-B2385 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2385 was determined to be 5 ug/ml. Western Blot: LS-B2385 detects a band of approximately 35kD in A20 cell lysates.. IHC, IHC-P (5 µg/ml), WB Immunohistochemistry: LS-B2385 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2385 was determined to be 5 ug/ml. Western Blot: LS-B2385 detects a band of approximately 35kD in A20 cell lysates.

Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

ORAI3

ORAI calcium release-activated calcium modulator 3

TMEM142C, protein orai-3, transmembrane protein 142C

Rabbit

IgG

Human

2°C to 8°C or -20°C

41116161