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ProductsBack/IHC-plus(tm) PBK / TOPK Antibody (clone 2C8)

IHC-plus(tm) PBK / TOPK Antibody (clone 2C8)

LS-B11896
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetPBK
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) PBK / TOPK Antibody (clone 2C8)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA (1:10000), Flo (1:200 - 1:400), IF (1:200 - 1:1000), IHC, IHC-P (1:50), WB (1:100 - 1:500)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2C8
  • Conjugate
    Unconjugated
  • Gene ID55872
  • Target name
    PBK
  • Target description
    PDZ binding kinase
  • Target synonyms
    CT84, HEL164, Nori-3, SPK, TOPK, lymphokine-activated killer T-cell-originated protein kinase, MAPKK-like protein kinase, T-LAK cell-originated protein kinase, cancer/testis antigen 84, epididymis luminal protein 164, serine/threonine protein kinase, spermatogenesis-related protein kinase
  • Host
    Mouse
  • Isotype
    IgG2b
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PBK using anti-PBK antibody (PB9310). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: mouse testis tissue lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PBK antigen affinity purified polyclonal antibody (Catalog # PB9310) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PBK at approximately 40 kDa. The expected band size for PBK is at 36 kDa.
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