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ProductsBack/IHC-plus(tm) PDCD4 Antibody (aa210-221)

IHC-plus(tm) PDCD4 Antibody (aa210-221)

LS-B9458
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Equine, Guinea Pig, Goat, Hamster, Human, Mammals, Monkey, Mouse, Rabbit, Rat, Xenopus
TargetPDCD4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) PDCD4 Antibody (aa210-221)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 50kD band observed in lysates of cell line HeLa (calculated MW of 50.2kD according to NP_001186421.1). Recommended concentration: 0.1-0.3 ug/ml. Tested in IHC-P only with human FFPE samples.. IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:128000), WB (0.1 - 0.3 µg/ml) Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 50kD band observed in lysates of cell line HeLa (calculated MW of 50.2kD according to NP_001186421.1). Recommended concentration: 0.1-0.3 ug/ml. Tested in IHC-P only with human FFPE samples.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID27250
  • Target name
    PDCD4
  • Target description
    programmed cell death 4
  • Target synonyms
    H731, programmed cell death protein 4, neoplastic transformation inhibitor protein, nuclear antigen H731, programmed cell death 4 (neoplastic transformation inhibitor), protein 197/15a
  • Host
    Goat
  • Reactivity
    Bovine, Canine, Chicken, Equine, Guinea Pig, Goat, Hamster, Human, Mammals, Monkey, Mouse, Rabbit, Rat, Xenopus
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PDCD4 using anti-PDCD4 antibody (A01105-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD4 antigen affinity purified polyclonal antibody (Catalog # A01105-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDCD4 at approximately 64 kDa. The expected band size for PDCD4 is at 52 kDa.
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