IHC-plus(tm) PDCD4 Antibody

LifeSpan BioSciences

IHC-plus(tm) PDCD4 Antibody

23

A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Pdcd4 from other sources has not been determined. The antibody reacts with Pdcd4

Immunohistochemistry: LS-B65 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B65 was determined to be 10 ug/ml.. ELISA (1:10000 - 1:100000), IHC, IHC-P (10 µg/ml), WB (1:1000 - 1:10000) Immunohistochemistry: LS-B65 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B65 was determined to be 10 ug/ml.

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PDCD4

programmed cell death 4

H731; neoplastic transformation inhibitor protein; nuclear antigen H731; programmed cell death 4 (neoplastic transformation inhibitor); programmed cell death protein 4; protein 197/15a

2°C to 8°C or -20°C

12352203