IHC-plus(tm) PDCD4 Antibody (phospho-Ser457)

LifeSpan BioSciences

IHC-plus(tm) PDCD4 Antibody (phospho-Ser457)

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This antibody is specific for human Pdcd4 protein phosphorylated at Ser457. A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Pdcd

Immunohistochemistry: LS-B487 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B487 was determined to be 1.25-2.5 ug/ml.. ELISA (1:400000), IHC, IHC-P (1.25 - 2.5 µg/ml), WB (1:500 - 1:2000) Immunohistochemistry: LS-B487 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B487 was determined to be 1.25-2.5 ug/ml.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

...

PDCD4

programmed cell death 4

H731; neoplastic transformation inhibitor protein; nuclear antigen H731; programmed cell death 4 (neoplastic transformation inhibitor); programmed cell death protein 4; protein 197/15a

Rabbit

IgG

Human, Mouse, Rat, Xenopus

-20°C,2°C to 8°C

12352203