IHC-plus(tm) PDCD4 Antibody (phospho-Ser457)

LifeSpan BioSciences

IHC-plus(tm) PDCD4 Antibody (phospho-Ser457)

23

Immunohistochemistry: LS-B487 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B487 was determined to be 1.25-2.5 ug/ml.. ELISA (1:400000), IHC, IHC-P (1.25 - 2.5 µg/ml), WB (1:500 - 1:2000) Immunohistochemistry: LS-B487 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B487 was determined to be 1.25-2.5 ug/ml.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

PDCD4

programmed cell death 4

H731, programmed cell death protein 4, neoplastic transformation inhibitor protein, nuclear antigen H731, programmed cell death 4 (neoplastic transformation inhibitor), protein 197/15a

Rabbit

IgG

Human, Mouse, Rat, Xenopus

-20°C,2°C to 8°C

41116161