More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/IHC-plus(tm) PYCARD / ASC / TMS1 Antibody

IHC-plus(tm) PYCARD / ASC / TMS1 Antibody

LS-B1393
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetPYCARD
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) PYCARD / ASC / TMS1 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    ASC antibody can be used for detection of ASC/TMS1 by Western blot at 1 ug/mL. Antibody can also be used for immunocytochemistry starting at 5 ug/mL and immunohistochemistry starting at 2.5 ug/mL.. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: 25 kDa. ELISA, ICC, IHC, IHC-P (5 µg/ml), WB (1 µg/ml) ASC antibody can be used for detection of ASC/TMS1 by Western blot at 1 ug/mL. Antibody can also be used for immunocytochemistry starting at 5 ug/mL and immunohistochemistry starting at 2.5 ug/mL.. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: 25 kDa
  • Applications
    Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID29108
  • Target name
    PYCARD
  • Target description
    PYD and CARD domain containing
  • Target synonyms
    ASC, CARD5, TMS, TMS-1, TMS1, apoptosis-associated speck-like protein containing a CARD, caspase recruitment domain-containing protein 5, target of methylation-induced silencing 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
HMBS Monoclonal AntibodyCAB11701
ReactivityHuman
TargetPYCARD
  • SizePrice
Western Blot analysis of 823 293T cells using ASC Polyclonal Antibody
Product group Antibodies
Cusabio
PYCARD AntibodyCSB-PA000940
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPYCARD
  • SizePrice
Product group Antibodies
Biomatik
PYCARD Polyclonal AntibodyCAC15806
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
TargetPYCARD
  • SizePrice
Figure 1. Western blot analysis of ASC/TMS1/PYCARD using anti-ASC/TMS1/PYCARD antibody (A00362-6). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASC/TMS1/PYCARD antigen affinity purified polyclonal antibody (Catalog # A00362-6) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASC/TMS1/PYCARD at approximately 24 kDa. The expected band size for ASC/TMS1/PYCARD is at 22 kDa.
Product group Antibodies
Boster Bio
Anti-ASC/TMS1/PYCARD Antibody Picoband(r)A00362-6-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetPYCARD
  • SizePrice
Product group Antibodies
RayBiotech
Anti-PYCARD Antibody144-01170
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman, Mouse, Rat
TargetPYCARD
  • SizePrice
Anti-TMS1/ASC Antibody (Biotin)
Product group Antibodies
Antibodies.com
Anti-TMS1/ASC Antibody (Biotin)A83387
ApplicationsWestern Blot, ELISA
ReactivityHuman
  • SizePrice
Western blot analysis of human and mouse cell lines using anti-Asc, pAb (AL177) (Prod. No. AG-25B-0006). Total protein extracts from various human (293-T, Jurkat, Raj, Ramos, BJAB, THP-1, U937, K562, Raw, HeLa) and mouse (EL-4, A20) cell lines were
Product group Antibodies
AdipoGen Life Sciences
anti-Asc, pAb (AL177)AG-25B-0006
ApplicationsFunctional Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetPYCARD
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
PYCARD / ASC / TMS1 AntibodyLS-C746971
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPYCARD
  • SizePrice
Product group Antibodies
MedChem Express
TMS1/ACS Antibody (YA033, Rabbit)HY-P80522
TargetPYCARD
  • SizePrice
Immunohistochemistry analysis in human spleen and skeletal muscle tissues using HPA054496 antibody. Corresponding PYCARD RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-PYCARD AntibodyHPA054496
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetPYCARD
  • SizePrice