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ProductsBack/IHC-plus(tm) RAD18 Antibody (clone 3H7)

IHC-plus(tm) RAD18 Antibody (clone 3H7)

LS-B15619
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRAD18
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) RAD18 Antibody (clone 3H7)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Cell lysate, Transfected lysate, Recombinant protein.. ELISA (1:100 - 1:2000), IF (25 µg/ml), IHC, IHC-P (1.5 - 10 µg/ml), RNAi, WB (1:500) Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Cell lysate, Transfected lysate, Recombinant protein.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3H7
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID56852
  • Target name
    RAD18
  • Target description
    RAD18 E3 ubiquitin protein ligase
  • Target synonyms
    RNF73, E3 ubiquitin-protein ligase RAD18, RAD18 homolog, RAD18, S. cerevisiae, homolog, RING finger protein 73, RING-type E3 ubiquitin transferase RAD18, hHR18, hRAD18, postreplication repair protein RAD18, postreplication repair protein hRAD18p
  • Host
    Mouse
  • Isotype
    IgG2b
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RAD18 using anti-RAD18 antibody (A01622-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAD18 antigen affinity purified polyclonal antibody (Catalog # A01622-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAD18 at approximately 75 kDa. The expected band size for RAD18 is at 56 kDa.
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